VESM
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Microscope Alignment
Sideport Alignment
The Filter Carousel should be removed.
A pen laser is set up on a vertical mount mounted on tghe stage. The laser should centered directly over a ground glass disk with a pin hole that is attached to the objective mount.
Adjust the microscope until center of beam is aligned with target.
Backport Alignment
The Filter Carousel should be installed, and #1 cube should be used.
Install an 8" vertical rod and a small thread rod on top. Only microscope side to side movement should be done.
Recheck Sideport alignment (sans Filter Carousel).
TuCam Alignment
Align the straight-through port, without cameras attached.
Do not have the TuCam dichroic in place.
Use filter cube 1 in the Filter Carousel.
Align beam to center of target.
Andor Zyla 4.2
TRIGGER MODE | FPS |
---|---|
External | 50.2978 |
External Exposure | 99.968 |
External Start | 50.3951 |
Internal | 50.3951 |
Software | 50.2978 |
Trigger FPS