Difference between revisions of "Microscopes"
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{| | {| | ||
|Microscope | |Microscope | ||
− | |Nikon TE2000 | + | |Nikon TE2000 |
+ | |- | ||
+ | |Types of Imaging | ||
+ | |Real Time Live Cell Imaging of fluorophores in modest to high quantity. | ||
+ | |- | ||
+ | |Software | ||
+ | |Metamorph | ||
|- | |- | ||
|Light Sources | |Light Sources | ||
Line 13: | Line 19: | ||
|- | |- | ||
|Spinning Disk Camera | |Spinning Disk Camera | ||
− | |QImaging Rolera-MGi, 512x512, '''14 bits''', | + | |QImaging Rolera-MGi, 512x512, '''14 bits''', 16um<sup>2</sup> pixels, 30 fps, '''QE: up to 90%''' |
|- | |- | ||
|Nipkow Spinning Disk | |Nipkow Spinning Disk | ||
Line 48: | Line 54: | ||
|Microscope | |Microscope | ||
|Zeiss | |Zeiss | ||
+ | |- | ||
+ | |Types of Imaging | ||
+ | |Slower than Real Time Imaging of fluorophores in modest to high quantity. | ||
+ | |- | ||
+ | |Software | ||
+ | |Metamorph | ||
|- | |- | ||
|Light Sources | |Light Sources | ||
Line 91: | Line 103: | ||
|Microscope | |Microscope | ||
| Olympus ix81 | | Olympus ix81 | ||
+ | |- | ||
+ | |Types of Imaging | ||
+ | |Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity. | ||
+ | |- | ||
+ | |Software | ||
+ | |[https://micro-manager.org/ μManager] | ||
|- | |- | ||
|Light Sources | |Light Sources | ||
− | |Halogen Lamp, X-Cite LED 4 Color ('''395nm''', 470nm,550nm, 640nm) | + | |Halogen Lamp, X-Cite LED 4 Color ('''395nm''', 470nm, 550nm, 640nm) |
|- | |- | ||
|Camera 1 | |Camera 1 | ||
Line 111: | Line 129: | ||
|- | |- | ||
|Focus Stability | |Focus Stability | ||
− | |'''Soon (pgFocus by Karl Bellvé)''' | + | |'''Soon ([http://big.umassmed.edu/wiki/index.php/PgFocus pgFocus] by Karl Bellvé)''' |
|- | |- | ||
|XYZ Motorized Stage | |XYZ Motorized Stage | ||
Line 134: | Line 152: | ||
|Microscope | |Microscope | ||
| Olympus ix71 | | Olympus ix71 | ||
+ | |- | ||
+ | |Types of Imaging | ||
+ | |Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane. | ||
+ | |- | ||
+ | |Software | ||
+ | |[https://micro-manager.org/ μManager] | ||
|- | |- | ||
|Light Sources | |Light Sources | ||
− | |Halogen Lamp, Lasers ( '''405nm''', 491nm, 561nm, 660nm ) | + | |Halogen Lamp, Lasers ('''405nm''', 491nm, 561nm, 660nm ) |
|- | |- | ||
|Camera | |Camera | ||
Line 151: | Line 175: | ||
|- | |- | ||
|Focus Stability | |Focus Stability | ||
− | |'''Yes (pgFocus by Karl Bellvé)''' | + | |'''Yes ([http://big.umassmed.edu/wiki/index.php/PgFocus pgFocus] by Karl Bellvé)''' |
|- | |- | ||
|XYZ Motorized Stage | |XYZ Motorized Stage | ||
Line 171: | Line 195: | ||
|Microscope | |Microscope | ||
| Zeiss Axio Observer Z1 | | Zeiss Axio Observer Z1 | ||
+ | |- | ||
+ | |Types of Imaging | ||
+ | |Slow Live Cell Imaging of fluorophores in high quantity. | ||
+ | |- | ||
+ | |Software | ||
+ | |[https://micro-manager.org/ μManager] | ||
|- | |- | ||
|Light Sources | |Light Sources |
Revision as of 17:59, 17 January 2017
Raffi's Spinning Disk Confocal Microscope
Microscope | Nikon TE2000 |
Types of Imaging | Real Time Live Cell Imaging of fluorophores in modest to high quantity. |
Software | Metamorph |
Light Sources | Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm) |
Wide Field Camera | Photometrics CoolSNAP HQ, 1392x1040, 12 bits, 6.45um2 pixels, QE: 55-62% |
Spinning Disk Camera | QImaging Rolera-MGi, 512x512, 14 bits, 16um2 pixels, 30 fps, QE: up to 90% |
Nipkow Spinning Disk | Yokogawa CSU-10 |
Differential interference contrast (DIC) | Yes |
TIRF | No |
Structured Light | No |
Focus Stability | Yes (Perfect Focus by Nikon) |
XYZ Motorized Stage | Yes |
Nano Z Stage | No |
Piezo Z | No |
Microablation System | Yes |
Doxsey's Spinning Disk Confocal Microscope
Microscope | Zeiss |
Types of Imaging | Slower than Real Time Imaging of fluorophores in modest to high quantity. |
Software | Metamorph |
Light Sources | Mercury Arc Lamp, Halogen Lamp, 3 Lasers (491nm, 561nm, 647nm) |
Wide-field Camera | None |
Spinning Disk Camera | Hamamatsu C4742-80, 1344x1024, 12 bits, 6.45um2 pixels, 8.8fps, QE : 60-72% |
Nipkow Spinning Disk | Yokogawa CSU-10 |
Differential interference contrast (DIC) | Unknown |
TIRF | No |
Structured Light | No |
Focus Stability | No |
XYZ Motorized Stage | Yes |
Nano Z Stage | No |
Piezo Z | Yes |
Microablation System | No |
VESM
Microscope | Olympus ix81 |
Types of Imaging | Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity. |
Software | μManager |
Light Sources | Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm, 550nm, 640nm) |
Camera 1 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70% |
Camera 2 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70% |
Differential interference contrast (DIC) | No |
TIRF | No |
Structured Light | No |
Focus Stability | Soon (pgFocus by Karl Bellvé) |
XYZ Motorized Stage | No |
Nano Z Stage | No |
Piezo Z | Yes |
Microablation System | No |
Notes | Housed in a Biosafety Level 2+ Room. Please see CDC Biosafety Level Criteria |
TESM
Microscope | Olympus ix71 |
Types of Imaging | Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane. |
Software | μManager |
Light Sources | Halogen Lamp, Lasers (405nm, 491nm, 561nm, 660nm ) |
Camera | Andor iXon 885 Em, 1004x1002,14 bits, 8um2,30fps, QE > 65% |
Differential interference contrast (DIC) | No |
TIRF | Yes |
Structured Light | Yes |
Focus Stability | Yes (pgFocus by Karl Bellvé) |
XYZ Motorized Stage | No |
Nano Z Stage | No |
Piezo Z | Yes |
Microablation System | No |
Multi-Well Microscope
Microscope | Zeiss Axio Observer Z1 |
Types of Imaging | Slow Live Cell Imaging of fluorophores in high quantity. |
Software | μManager |
Light Sources | Halogen Lamp, Xenon Arc Lamp |
Camera | Andor Clara, 1392x1040, 14 & 16 bit, 6.45um2, 11fps, QE 40-60% |
Differential interference contrast (DIC) | No |
TIRF | No |
Structured Light | No |
Focus Stability | Yes (Definite Focus by Zeiss) |
XYZ Motorized Stage | Yes |
Nano Z Stage | No |
Piezo Z | No |
Microablation System | No |
Notes | This microscope is dedicated to imaging 96 well plates. |