Difference between revisions of "Microscopes"

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{|
 
{|
 
|Microscope
 
|Microscope
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|Nikon TE2000
+
|Nikon TE2000
 +
|-
 +
|Types of Imaging
 +
|Real Time Live Cell Imaging of fluorophores in modest to high quantity.
 +
|-
 +
|Software
 +
|Metamorph
 
|-
 
|-
 
|Light Sources
 
|Light Sources
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|-
 
|-
 
|Spinning Disk Camera
 
|Spinning Disk Camera
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|QImaging Rolera-MGi, 512x512, '''14 bits''', 16um2, 30 fps, '''QE: up to 90%'''
+
|QImaging Rolera-MGi, 512x512, '''14 bits''', 16um<sup>2</sup> pixels, 30 fps, '''QE: up to 90%'''
 
|-
 
|-
 
|Nipkow Spinning Disk
 
|Nipkow Spinning Disk
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|Microscope
 
|Microscope
 
|Zeiss
 
|Zeiss
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|-
 +
|Types of Imaging
 +
|Slower than Real Time Imaging of fluorophores in modest to high quantity.
 +
|-
 +
|Software
 +
|Metamorph
 
|-
 
|-
 
|Light Sources
 
|Light Sources
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|Microscope
 
|Microscope
 
| Olympus ix81
 
| Olympus ix81
 +
|-
 +
|Types of Imaging
 +
|Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity.
 +
|-
 +
|Software
 +
|[https://micro-manager.org/ &mu;Manager]
 
|-
 
|-
 
|Light Sources
 
|Light Sources
−
|Halogen Lamp, X-Cite LED 4 Color ('''395nm''', 470nm,550nm, 640nm)
+
|Halogen Lamp, X-Cite LED 4 Color ('''395nm''', 470nm, 550nm, 640nm)
 
|-
 
|-
 
|Camera 1
 
|Camera 1
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|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|'''Soon (pgFocus by Karl Bellvé)'''
+
|'''Soon ([http://big.umassmed.edu/wiki/index.php/PgFocus pgFocus] by Karl Bellvé)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
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|Microscope
 
|Microscope
 
| Olympus ix71
 
| Olympus ix71
 +
|-
 +
|Types of Imaging
 +
|Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane.
 +
|-
 +
|Software
 +
|[https://micro-manager.org/ &mu;Manager]
 
|-
 
|-
 
|Light Sources
 
|Light Sources
−
|Halogen Lamp, Lasers ( '''405nm''', 491nm, 561nm, 660nm )
+
|Halogen Lamp, Lasers ('''405nm''', 491nm, 561nm, 660nm )
 
|-
 
|-
 
|Camera
 
|Camera
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|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|'''Yes (pgFocus by Karl Bellvé)'''
+
|'''Yes ([http://big.umassmed.edu/wiki/index.php/PgFocus pgFocus] by Karl Bellvé)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
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|Microscope
 
|Microscope
 
| Zeiss Axio Observer Z1
 
| Zeiss Axio Observer Z1
 +
|-
 +
|Types of Imaging
 +
|Slow Live Cell Imaging of fluorophores in high quantity.
 +
|-
 +
|Software
 +
|[https://micro-manager.org/ &mu;Manager]
 
|-
 
|-
 
|Light Sources
 
|Light Sources

Revision as of 17:59, 17 January 2017

Raffi's Spinning Disk Confocal Microscope

Microscope Nikon TE2000
Types of Imaging Real Time Live Cell Imaging of fluorophores in modest to high quantity.
Software Metamorph
Light Sources Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm)
Wide Field Camera Photometrics CoolSNAP HQ, 1392x1040, 12 bits, 6.45um2 pixels, QE: 55-62%
Spinning Disk Camera QImaging Rolera-MGi, 512x512, 14 bits, 16um2 pixels, 30 fps, QE: up to 90%
Nipkow Spinning Disk Yokogawa CSU-10
Differential interference contrast (DIC) Yes
TIRF No
Structured Light No
Focus Stability Yes (Perfect Focus by Nikon)
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z No
Microablation System Yes

Doxsey's Spinning Disk Confocal Microscope

Microscope Zeiss
Types of Imaging Slower than Real Time Imaging of fluorophores in modest to high quantity.
Software Metamorph
Light Sources Mercury Arc Lamp, Halogen Lamp, 3 Lasers (491nm, 561nm, 647nm)
Wide-field Camera None
Spinning Disk Camera Hamamatsu C4742-80, 1344x1024, 12 bits, 6.45um2 pixels, 8.8fps, QE : 60-72%
Nipkow Spinning Disk Yokogawa CSU-10
Differential interference contrast (DIC) Unknown
TIRF No
Structured Light No
Focus Stability No
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z Yes
Microablation System No

VESM

Microscope Olympus ix81
Types of Imaging Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity.
Software μManager
Light Sources Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm, 550nm, 640nm)
Camera 1 Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70%
Camera 2 Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70%
Differential interference contrast (DIC) No
TIRF No
Structured Light No
Focus Stability Soon (pgFocus by Karl Bellvé)
XYZ Motorized Stage No
Nano Z Stage No
Piezo Z Yes
Microablation System No
Notes Housed in a Biosafety Level 2+ Room. Please see CDC Biosafety Level Criteria

TESM

Microscope Olympus ix71
Types of Imaging Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane.
Software μManager
Light Sources Halogen Lamp, Lasers (405nm, 491nm, 561nm, 660nm )
Camera Andor iXon 885 Em, 1004x1002,14 bits, 8um2,30fps, QE > 65%
Differential interference contrast (DIC) No
TIRF Yes
Structured Light Yes
Focus Stability Yes (pgFocus by Karl Bellvé)
XYZ Motorized Stage No
Nano Z Stage No
Piezo Z Yes
Microablation System No

Multi-Well Microscope

Microscope Zeiss Axio Observer Z1
Types of Imaging Slow Live Cell Imaging of fluorophores in high quantity.
Software μManager
Light Sources Halogen Lamp, Xenon Arc Lamp
Camera Andor Clara, 1392x1040, 14 & 16 bit, 6.45um2, 11fps, QE 40-60%
Differential interference contrast (DIC) No
TIRF No
Structured Light No
Focus Stability Yes (Definite Focus by Zeiss)
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z No
Microablation System No
Notes This microscope is dedicated to imaging 96 well plates.
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