Difference between revisions of "Microscopes"

From Wiki
Jump to navigation Jump to search
Line 7: Line 7:
 
|-
 
|-
 
|Light Sources
 
|Light Sources
−
|Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm)
+
|Xeon Arc Lamp, Halogen Lamp, 4 Lasers ('''405nm''', 488nm, 561nm, 636nm)
 
|-
 
|-
 
|Wide Field Camera
 
|Wide Field Camera
Line 13: Line 13:
 
|-
 
|-
 
|Spinning Disk Camera
 
|Spinning Disk Camera
−
|QImaging Rolera-MGi, 512x512, 14 bits, 16um2, 30 fps, QE: up to 90%
+
|QImaging Rolera-MGi, 512x512, '''14 bits''', 16um2, 30 fps, '''QE: up to 90%'''
 
|-
 
|-
 
|Nipkow Spinning Disk
 
|Nipkow Spinning Disk
Line 19: Line 19:
 
|-
 
|-
 
|Differential interference contrast (DIC)
 
|Differential interference contrast (DIC)
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|TIRF
 
|TIRF
Line 28: Line 28:
 
|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|Yes (Perfect Focus by Nikon)
+
|'''Yes (Perfect Focus by Nikon)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Nano Z Stage
 
|Nano Z Stage
Line 40: Line 40:
 
|-
 
|-
 
|Microablation System
 
|Microablation System
−
|Yes
+
|'''Yes'''
 
|}
 
|}
  
Line 74: Line 74:
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Nano Z Stage
 
|Nano Z Stage
Line 80: Line 80:
 
|-
 
|-
 
|Piezo Z
 
|Piezo Z
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Microablation System
 
|Microablation System
Line 93: Line 93:
 
|-
 
|-
 
|Light Sources
 
|Light Sources
−
|Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm,550nm, 640nm)
+
|Halogen Lamp, X-Cite LED 4 Color ('''395nm''', 470nm,550nm, 640nm)
 
|-
 
|-
 
|Camera 1
 
|Camera 1
−
|Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um<sup>2</sup>, 100fps, QE >70%
+
|Andor Zyla CMOS 4.2, '''2048x2048''', '''12 & 16 bit modes''', 6.45um<sup>2</sup>, '''100fps''', QE >70%
 
|-
 
|-
 
|Camera 2
 
|Camera 2
−
|Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um<sup>2</sup>, 100fps, QE >70%
+
|Andor Zyla CMOS 4.2, '''2048x2048''', '''12 & 16 bit modes''', 6.45um<sup>2</sup>, '''100fps''', QE >70%
 
|-
 
|-
 
|Differential interference contrast (DIC)
 
|Differential interference contrast (DIC)
Line 111: Line 111:
 
|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|Soon (pgFocus by Karl Bellvé)
+
|'''Soon (pgFocus by Karl Bellvé)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
Line 133: Line 133:
 
|-
 
|-
 
|Light Sources
 
|Light Sources
−
|Halogen Lamp, Lasers ( 405nm, 491nm, 561nm, 660nm )
+
|Halogen Lamp, Lasers ( '''405nm''', 491nm, 561nm, 660nm )
 
|-
 
|-
 
|Camera
 
|Camera
−
|Andor iXon 885 Em, 1004x1002,14 bits, 8um<sup>2</sup>,30fps, QE > 65%
+
|Andor iXon 885 Em, 1004x1002,'''14 bits''', 8um<sup>2</sup>,30fps, QE > 65%
 
|-
 
|-
 
|Differential interference contrast (DIC)
 
|Differential interference contrast (DIC)
Line 142: Line 142:
 
|-
 
|-
 
|TIRF
 
|TIRF
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Structured Light
 
|Structured Light
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|Yes (pgFocus by Karl Bellvé)
+
|'''Yes (pgFocus by Karl Bellvé)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
Line 157: Line 157:
 
|-
 
|-
 
|Piezo Z
 
|Piezo Z
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Microablation System
 
|Microablation System
Line 185: Line 185:
 
|-
 
|-
 
|Focus Stability
 
|Focus Stability
−
|Yes (Definite Focus by Zeiss)
+
|'''Yes (Definite Focus by Zeiss)'''
 
|-
 
|-
 
|XYZ Motorized Stage
 
|XYZ Motorized Stage
−
|Yes
+
|'''Yes'''
 
|-
 
|-
 
|Nano Z Stage
 
|Nano Z Stage

Revision as of 16:44, 17 January 2017

Raffi's Spinning Disk Confocal Microscope

Microscope Nikon TE2000
Light Sources Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm)
Wide Field Camera Photometrics CoolSNAP HQ, 1392x1040, 12 bits, 6.45um2 pixels, QE: 55-62%
Spinning Disk Camera QImaging Rolera-MGi, 512x512, 14 bits, 16um2, 30 fps, QE: up to 90%
Nipkow Spinning Disk Yokogawa CSU-10
Differential interference contrast (DIC) Yes
TIRF No
Structured Light No
Focus Stability Yes (Perfect Focus by Nikon)
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z No
Microablation System Yes

Doxsey's Spinning Disk Confocal Microscope

Microscope Zeiss
Light Sources Mercury Arc Lamp, Halogen Lamp, 3 Lasers (491nm, 561nm, 647nm)
Wide-field Camera None
Spinning Disk Camera Hamamatsu C4742-80, 1344x1024, 12 bits, 6.45um2 pixels, 8.8fps, QE : 60-72%
Nipkow Spinning Disk Yokogawa CSU-10
Differential interference contrast (DIC) Unknown
TIRF No
Structured Light No
Focus Stability No
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z Yes
Microablation System No

VESM

Microscope Olympus ix81
Light Sources Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm,550nm, 640nm)
Camera 1 Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70%
Camera 2 Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70%
Differential interference contrast (DIC) No
TIRF No
Structured Light No
Focus Stability Soon (pgFocus by Karl Bellvé)
XYZ Motorized Stage No
Nano Z Stage No
Piezo Z Yes
Microablation System No

TESM

Microscope Olympus ix71
Light Sources Halogen Lamp, Lasers ( 405nm, 491nm, 561nm, 660nm )
Camera Andor iXon 885 Em, 1004x1002,14 bits, 8um2,30fps, QE > 65%
Differential interference contrast (DIC) No
TIRF Yes
Structured Light Yes
Focus Stability Yes (pgFocus by Karl Bellvé)
XYZ Motorized Stage No
Nano Z Stage No
Piezo Z Yes
Microablation System No

Multi-Well Microscope

Microscope Zeiss Axio Observer Z1
Light Sources Halogen Lamp, Xenon Arc Lamp
Camera Andor Clara, 1392x1040, 14 & 16 bit, 6.45um2, 11fps, QE 40-60%
Differential interference contrast (DIC) No
TIRF No
Structured Light No
Focus Stability Yes (Definite Focus by Zeiss)
XYZ Motorized Stage Yes
Nano Z Stage No
Piezo Z No
Microablation System No
Retrieved from "http://big.umassmed.edu/wiki/index.php?title=Microscopes&oldid=384"