Difference between revisions of "Microscopes"
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| Line 6: | Line 6: | ||
|Real Time Live Cell Imaging of fluorophores in modest to high quantity. | |Real Time Live Cell Imaging of fluorophores in modest to high quantity. | ||
|- | |- | ||
| − | | | + | |Example Fluorophores |
| − | |Hoechst, DAPI, GFP, RFP, Cy3, Cy5 | + | |Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 633, Cy5 |
|- | |- | ||
|Microscope | |Microscope | ||
| Line 58: | Line 58: | ||
|Slower than Real Time Imaging of fluorophores in modest to high quantity. | |Slower than Real Time Imaging of fluorophores in modest to high quantity. | ||
|- | |- | ||
| − | | | + | |Example Fluorophores |
| − | |GFP, RFP, Cy3, Cy5 | + | |Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
|- | |- | ||
|Microscope | |Microscope | ||
| Line 110: | Line 110: | ||
|Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity. | |Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity. | ||
|- | |- | ||
| − | | | + | |Example Fluorophores |
| − | |Hoechst, DAPI, GFP, RFP, Cy3, Cy5 | + | |Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 632, Cy5 |
|- | |- | ||
|Microscope | |Microscope | ||
| Line 162: | Line 162: | ||
|Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane. | |Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane. | ||
|- | |- | ||
| − | | | + | |Example Fluorophores |
| − | |Hoechst, DAPI, GFP, RFP, Cy3, Cy5 | + | |Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 650, Cy5 |
|- | |- | ||
|Microscope | |Microscope | ||
| Line 209: | Line 209: | ||
|- | |- | ||
|Common Fluorophores | |Common Fluorophores | ||
| − | |Hoechst, DAPI, GFP, RFP, Cy3, Cy5 | + | |Hoechst, DAPI, FITC, GFP, RFP, Cy3, Cy5 |
|- | |- | ||
|Microscope | |Microscope | ||
Revision as of 18:19, 17 January 2017
Raffi's Spinning Disk Confocal Microscope
| Types of Imaging | Real Time Live Cell Imaging of fluorophores in modest to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 633, Cy5 |
| Microscope | Nikon TE2000 |
| Software | Metamorph |
| Light Sources | Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm) |
| Wide Field Camera | Photometrics CoolSNAP HQ, 1392x1040, 12 bits, 6.45um2 pixels, QE: 55-62% |
| Spinning Disk Camera | QImaging Rolera-MGi, 512x512, 14 bits, 16um2 pixels, 30 fps, QE: up to 90% |
| Nipkow Spinning Disk | Yokogawa CSU-10 |
| Differential interference contrast (DIC) | Yes |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Yes (Perfect Focus by Nikon) |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | No |
| Microablation System | Yes |
Doxsey's Spinning Disk Confocal Microscope
| Types of Imaging | Slower than Real Time Imaging of fluorophores in modest to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
| Microscope | Zeiss |
| Software | Metamorph |
| Light Sources | Mercury Arc Lamp, Halogen Lamp, 3 Lasers (491nm, 561nm, 647nm) |
| Wide-field Camera | None |
| Spinning Disk Camera | Hamamatsu C4742-80, 1344x1024, 12 bits, 6.45um2 pixels, 8.8fps, QE : 60-72% |
| Nipkow Spinning Disk | Yokogawa CSU-10 |
| Differential interference contrast (DIC) | Unknown |
| TIRF | No |
| Structured Light | No |
| Focus Stability | No |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
VESM
| Types of Imaging | Faster than Real Time Live Cell Imaging of two fluorophores simultaneously in low to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 632, Cy5 |
| Microscope | Olympus ix81 |
| Software | μManager |
| Light Sources | Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm, 550nm, 640nm) |
| Camera 1 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70% |
| Camera 2 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45um2, 100fps, QE >70% |
| Differential interference contrast (DIC) | No |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Soon (pgFocus by Karl Bellvé) |
| XYZ Motorized Stage | No |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
| Notes | Housed in a Biosafety Level 2+ Room. Please see CDC Biosafety Level Criteria |
TESM
| Types of Imaging | Real Time Live Cell Imaging of fluorophores in low to high quantity near or in the plasma membrane. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 650, Cy5 |
| Microscope | Olympus ix71 |
| Software | μManager |
| Light Sources | Halogen Lamp, Lasers (405nm, 491nm, 561nm, 660nm ) |
| Camera | Andor iXon 885 Em, 1004x1002,14 bits, 8um2,30fps, QE > 65% |
| Differential interference contrast (DIC) | No |
| TIRF | Yes |
| Structured Light | Yes |
| Focus Stability | Yes (pgFocus by Karl Bellvé) |
| XYZ Motorized Stage | No |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
Multi-Well Microscope
| Types of Imaging | Slow Live Cell Imaging of fluorophores in high quantity optionally 96 well plates. |
| Common Fluorophores | Hoechst, DAPI, FITC, GFP, RFP, Cy3, Cy5 |
| Microscope | Zeiss Axio Observer Z1 |
| Software | μManager |
| Light Sources | Halogen Lamp, Xenon Arc Lamp |
| Camera | Andor Clara, 1392x1040, 14 & 16 bit, 6.45um2, 11fps, QE 40-60% |
| Differential interference contrast (DIC) | No |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Yes (Definite Focus by Zeiss) |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | No |
| Microablation System | No |