Difference between revisions of "Microscopes"
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Virus Epifluorescence
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|Types of Imaging | |Types of Imaging | ||
| − | |Real | + | |Real time 2D, with slower 3D, live cell imaging of fluorophores in modest to high quantity |
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|Example Fluorophores | |Example Fluorophores | ||
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|Types of Imaging | |Types of Imaging | ||
| − | |Slower than | + | |Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
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|Example Fluorophores | |Example Fluorophores | ||
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{| | {| | ||
|Types of Imaging | |Types of Imaging | ||
| − | |Slower than | + | |Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
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|Example Fluorophores | |Example Fluorophores | ||
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{| | {| | ||
|Types of Imaging | |Types of Imaging | ||
| − | |Slower than | + | |Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
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|Example Fluorophores | |Example Fluorophores | ||
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|Types of Imaging | |Types of Imaging | ||
| − | |Faster than | + | |Faster than real time 2D/3D live cell imaging of two fluorophores simultaneously in low to high quantity. |
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|Example Fluorophores | |Example Fluorophores | ||
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{| | {| | ||
|Types of Imaging | |Types of Imaging | ||
| − | |Real | + | |Real time 2D/3D live cell imaging of fluorophores in low to high quantity near or in the plasma membrane. |
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|Example Fluorophores | |Example Fluorophores | ||
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|Types of Imaging | |Types of Imaging | ||
| − | |Slow | + | |Slow 2D/3D live cell imaging of fluorophores in high quantity optionally using 96 well plates. |
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|Common Fluorophores | |Common Fluorophores | ||
Revision as of 15:15, 18 January 2017
Microscopes in Molecular Medicine
Raffi Aroian's Spinning Disk Confocal Microscope
| Types of Imaging | Real time 2D, with slower 3D, live cell imaging of fluorophores in modest to high quantity |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 633, Cy5 |
| Microscope | Nikon TE2000 |
| Software | Metamorph |
| Light Sources | Xeon Arc Lamp, Halogen Lamp, 4 Lasers (405nm, 488nm, 561nm, 636nm) |
| Wide Field Camera | Photometrics CoolSNAP HQ, 1392x1040, 12 bits, 6.45μm2 pixels, QE: 55-62% |
| Spinning Disk Camera | QImaging Rolera-MGi, 512x512, 14 bits, 16μm2 pixels, 30 fps, QE: up to 90% |
| Nipkow Spinning Disk | Yokogawa CSU-10 |
| Differential interference contrast (DIC) | Yes |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Yes (Perfect Focus by Nikon) |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | No |
| Microablation System | Yes |
Stephen Doxsey's Spinning Disk Confocal Microscope
| Types of Imaging | Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
| Example Fluorophores | FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
| Microscope | Zeiss |
| Software | Metamorph |
| Light Sources | Mercury Arc Lamp, Halogen Lamp, 3 Lasers (491nm, 561nm, 647nm) |
| Wide-field Camera | None |
| Spinning Disk Camera | Hamamatsu C4742-80, 1344x1024, 12 bits, 6.45μm2 pixels, 8.8fps, QE : 60-72% |
| Nipkow Spinning Disk | Yokogawa CSU-10 |
| Differential interference contrast (DIC) | Unknown |
| TIRF | No |
| Structured Light | No |
| Focus Stability | No |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
Stephen Doxsey's Epifluorescence Microscope
| Types of Imaging | Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
| Microscope | Zeiss Axio Observer D1 |
| Software | Metamorph |
| Light Sources | X-Cite 120LED (4 Color), Halogen Lamp |
| Wide-field Camera | Digital CCD Hamamatsu Orca Flash 4, 2048x2048, 6.45μm2 pixels, 16 bit (2 * 11 bit D/A merged), QE < 82% |
| Differential interference contrast (DIC) | Yes |
| TIRF | No |
| Structured Light | No |
| Focus Stability | No |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | No |
| Microablation System | No |
Paul Furcinitti's Epifluorescence Microscope
| Types of Imaging | Slower than real time 2D/3D imaging of fluorophores in modest to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
| Microscope | Olympus IX70 |
| Software | Metamorph |
| Light Sources | Halogen Lamp, Mercury Arc Lamp, Xenon Arc Lamp |
| Wide-field Camera | Roper Scientific Coolsnap HQ, 1392x1040, 6.45μm2 pixels, 12 bit, QE < 65% |
| Differential interference contrast (DIC) | No |
| TIRF | No |
| Structured Light | No |
| Focus Stability | No |
| XYZ Motorized Stage | No |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
Virus Epifluorescence Structured Light Microscope (VESM)
| Types of Imaging | Faster than real time 2D/3D live cell imaging of two fluorophores simultaneously in low to high quantity. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 633, Alexa 632, Cy5 |
| Microscope | Olympus ix81 |
| Software | μManager |
| Light Sources | Halogen Lamp, X-Cite LED 4 Color (395nm, 470nm, 550nm, 640nm) |
| Camera 1 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45μm2, 100fps, QE >70% |
| Camera 2 | Andor Zyla CMOS 4.2, 2048x2048, 12 & 16 bit modes, 6.45μm2, 100fps, QE >70% |
| Differential interference contrast (DIC) | No |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Soon (pgFocus by Karl Bellvé) |
| XYZ Motorized Stage | No |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
| Notes | Housed in a Biosafety Level 2+ Room. Please see CDC Biosafety Level Criteria |
TIRF EpiFluorescence Structured Light Microscope (TESM)
| Types of Imaging | Real time 2D/3D live cell imaging of fluorophores in low to high quantity near or in the plasma membrane. |
| Example Fluorophores | Hoechst, DAPI, FITC, DyLight 488, Alexa 488, GFP, DyLight 550, Alexa 555, RFP, Cy3, DyLight 650, Alexa 647, Cy5 |
| Microscope | Olympus ix71 |
| Software | μManager |
| Light Sources | Halogen Lamp, Lasers (405nm, 491nm, 561nm, 660nm ) |
| Camera | Andor iXon 885 Em, 1004x1002,14 bits, 8μm2,30fps, QE > 65% |
| Differential interference contrast (DIC) | No |
| TIRF | Yes |
| Structured Light | Yes |
| Focus Stability | Yes (pgFocus by Karl Bellvé) |
| XYZ Motorized Stage | No |
| Nano Z Stage | No |
| Piezo Z | Yes |
| Microablation System | No |
Multi-Well Microscope
| Types of Imaging | Slow 2D/3D live cell imaging of fluorophores in high quantity optionally using 96 well plates. |
| Common Fluorophores | Hoechst, DAPI, FITC, GFP, RFP, Cy3, Cy5 |
| Microscope | Zeiss Axio Observer Z1 |
| Software | μManager |
| Light Sources | Halogen Lamp, Xenon Arc Lamp |
| Camera | Andor Clara, 1392x1040, 14 & 16 bit, 6.45μm2, 11fps, QE 40-60% |
| Differential interference contrast (DIC) | No |
| TIRF | No |
| Structured Light | No |
| Focus Stability | Yes (Definite Focus by Zeiss) |
| XYZ Motorized Stage | Yes |
| Nano Z Stage | No |
| Piezo Z | No |
| Microablation System | No |