TESM

TESM

TESM stands for TIRF Epi-fluorescence Structured Light Microscope. Some specifications can be found here

TESM Startup

Power

TESM is turned on by the two power switches at the bottom of the equipment rack to the left of the optical table. The order of which you turn on doesn't matter.

Lasers

The lasers should be power cycled once they have power. The keys should moved to the horizontal position, and then moved to the vertical position. It can take as much as 5 minutes for them to go through their start -up cycle. During this time, they will ignore any commands from the computer. They will default to full power, about 100mW.

The keys should be moved back to the horizontal position when you are finished using TESM for the day.

Computer

Log into TESM's computer, named Germanium, with your BIG credentials. Either start micromanager via an icon on your desktop, or open a terminal and type:

micromanager 

If that fails, you can:

cd /usr/local/Imagej 
./run 

TIRF or Structured Light

TESM has two imaging modes: TIRF and Structured Light. The Structured Light pathway is labelled EPI on the uniblitz. Only one of the two modes should be active at a given time.

Normally, the imaging mode is selected by the computer, but you may override the computer by using two most left toggles on the top UNIBLITZ shutter driver.

Laser Shutters

TESM has 4 lasers: 405nm, 491nm, 561nm, and 660nm.

Each laser shutter can be manually opened by moving their respective toggle switch up. Remember, either the TIRF or the EPI (Structured Light) shutter must be open as well.

Normally, the which laser is being used is selected by the computer, but you may override the computer by the appropriate toggle on the UNIBLITZ shutter driver.