Difference between revisions of "Two Cam FRET Microscope"
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| − | [[File:Two-cam.jpg|right]] | + | [[File:Two-cam.jpg|333x360px|right]] |
| − | This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already | + | This system is under development and will change. |
| + | |||
| + | This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already installed on the microscope that matches the dichroic and excitation filters below. Additional emission filter can be installed in front of each camera. | ||
== 3D printed Adapter == | == 3D printed Adapter == | ||
| Line 12: | Line 14: | ||
2 x [https://www.ptgrey.com/chameleon3-32-mp-mono-usb3-vision-sonyimx265 Chameleon3] | 2 x [https://www.ptgrey.com/chameleon3-32-mp-mono-usb3-vision-sonyimx265 Chameleon3] | ||
| + | |||
| + | Why the Chameleon3? | ||
| + | |||
| + | {| class="wikitable" | ||
| + | |Resolution | ||
| + | |2048x1536 | ||
| + | |- | ||
| + | |FPS | ||
| + | |55 | ||
| + | |- | ||
| + | |QE | ||
| + | |71% at 525nm | ||
| + | |- | ||
| + | |Dark Noise (e-) | ||
| + | |2.89 | ||
| + | |- | ||
| + | |Price | ||
| + | |$465 | ||
| + | |} | ||
| + | |||
| + | == Camera Heat Sinks == | ||
| + | [[File:Chameleon3-heatsink.jpg|right|320x240px]] | ||
| + | |||
| + | These will require slight machining. | ||
| + | |||
| + | 4 x [http://a.co/d/5193Liu M.2 22 X 110 SSD Aluminum Heat sink] | ||
| + | |||
| + | Picture on the right is of two Chameleon3s. The top one has the heat sink installed, and other does not | ||
== Excitation Filters == | == Excitation Filters == | ||
| Line 29: | Line 59: | ||
== Thorlab Parts == | == Thorlab Parts == | ||
| − | 30 mm Cage Cube: | + | 30 mm Cage Cube: |
1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH CM1-DCH] | 1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH CM1-DCH] | ||
| − | Snap-On Plastic Dust Cap: | + | Snap-On Plastic Dust Cap: |
1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B SM1EC2B] | 1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B SM1EC2B] | ||
| − | SM1 Lens Tube: | + | SM1 Lens Tube: |
2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03 SM1L03] | 2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03 SM1L03] | ||
| − | SM1 Adapter with External C-Mount Threads | + | SM1 Adapter with External C-Mount Threads: |
2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39 SM1A39] | 2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39 SM1A39] | ||
| − | Magnetic Post Base: | + | Magnetic Post Base: |
2x [https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E PH2E] | 2x [https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E PH2E] | ||
| + | == Micromanager == | ||
| + | |||
| + | We are using Micromanager with the [https://micro-manager.org/wiki/Point_Grey_Research Pt Grey] driver in combination with the [https://micro-manager.org/wiki/Utilities#Multi-Camera Multi-Camera] mode . | ||
| + | |||
| + | == Discussion == | ||
| + | |||
| + | We are seeing a 1.3mm shift on the X axis between the two cameras. This is due to the dichroic. We are looking at Thorlabs [https://www.thorlabs.com/thorproduct.cfm?partnumber=ST1XY-A ST1XY-A]. | ||
Latest revision as of 17:58, 6 September 2018
This system is under development and will change.
This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already installed on the microscope that matches the dichroic and excitation filters below. Additional emission filter can be installed in front of each camera.
3D printed Adapter
Two Camera Adapter:
http://a360.co/2IGYe2w
Cameras
2 x Chameleon3
Why the Chameleon3?
| Resolution | 2048x1536 |
| FPS | 55 |
| QE | 71% at 525nm |
| Dark Noise (e-) | 2.89 |
| Price | $465 |
Camera Heat Sinks
These will require slight machining.
4 x M.2 22 X 110 SSD Aluminum Heat sink
Picture on the right is of two Chameleon3s. The top one has the heat sink installed, and other does not
Excitation Filters
ET436/20x
ET505/20x
Dichroic
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
NOTE: A 3mm dichroic does not fit in the CM1-DCH cube.
Thorlab Parts
30 mm Cage Cube:
1 x CM1-DCH
Snap-On Plastic Dust Cap:
1 x SM1EC2B
SM1 Lens Tube:
2 x SM1L03
SM1 Adapter with External C-Mount Threads:
2 x SM1A39
Magnetic Post Base:
2x PH2E
Micromanager
We are using Micromanager with the Pt Grey driver in combination with the Multi-Camera mode .
Discussion
We are seeing a 1.3mm shift on the X axis between the two cameras. This is due to the dichroic. We are looking at Thorlabs ST1XY-A.
Notes:
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.