Difference between revisions of "Two Cam FRET Microscope"
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This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already instead on the microscope that matches the dichroic and excitation filters below. Addition emission filter can be installed in front of each camera. | This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already instead on the microscope that matches the dichroic and excitation filters below. Addition emission filter can be installed in front of each camera. | ||
Revision as of 15:15, 30 August 2018
This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already instead on the microscope that matches the dichroic and excitation filters below. Addition emission filter can be installed in front of each camera.
3D printed Adapter
Two Camera Adapter:
http://a360.co/2IGYe2w
Cameras
2 x Chameleon3
Excitation Filters
ET436/20x
ET505/20x
CM1-DCH Dichroic
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
NOTE: A 3mm dichroic does not fit in the CM1-DCH cube.
Thorlab Parts
30 mm Cage Cube: 1 x
1 x CM1-DCH
Snap-On Plastic Dust Cap: 1 x
1 x SM1EC2B
SM1 Lens Tube: 2 x
2 x SM1L03
SM1 Adapter with External C-Mount Threads 2 x
2 x SM1A39
Magnetic Post Base: 2 x
2x PH2E
Notes:
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.