Difference between revisions of "Two Cam FRET Microscope"
(Created page with " This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M. This is to make a two camera CFP/YFP fret imaging microscope. Two Camera Adapter: http://a360....") |
|||
| Line 4: | Line 4: | ||
This is to make a two camera CFP/YFP fret imaging microscope. | This is to make a two camera CFP/YFP fret imaging microscope. | ||
| + | An adapter needs to be 3D printed. | ||
Two Camera Adapter: | Two Camera Adapter: | ||
| Line 9: | Line 10: | ||
| − | Need an "Imaging Splitting" Dichroic: | + | Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube: |
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr | 25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr | ||
| − | + | A 3mm dichroic does not fit in the thorlab cube we chose. | |
| + | |||
| + | Thorlab Parts: | ||
| + | |||
| + | 30 mm Cage Cube: 1 x | ||
| + | https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH | ||
| + | |||
| + | Snap-On Plastic Dust Cap: 1 x | ||
| + | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B | ||
| + | |||
| + | SM1 Lens Tube: 2 x | ||
| + | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03 | ||
| + | |||
| + | SM1 Adapter with External C-Mount Threads 2 x | ||
| + | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39 | ||
| + | |||
| + | Magnetic Post Base: 2 x | ||
| + | https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E | ||
| + | |||
Revision as of 14:17, 30 August 2018
This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M.
This is to make a two camera CFP/YFP fret imaging microscope.
An adapter needs to be 3D printed.
Two Camera Adapter:
http://a360.co/2IGYe2w
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
A 3mm dichroic does not fit in the thorlab cube we chose.
Thorlab Parts:
30 mm Cage Cube: 1 x https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH
Snap-On Plastic Dust Cap: 1 x https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B
SM1 Lens Tube: 2 x https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03
SM1 Adapter with External C-Mount Threads 2 x https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39
Magnetic Post Base: 2 x https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E
Notes:
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.