Difference between revisions of "Two Cam FRET Microscope"
| Line 4: | Line 4: | ||
This is to make a two camera CFP/YFP fret imaging microscope. | This is to make a two camera CFP/YFP fret imaging microscope. | ||
| − | + | == 3D printed Adapter == | |
Two Camera Adapter: | Two Camera Adapter: | ||
http://a360.co/2IGYe2w | http://a360.co/2IGYe2w | ||
| + | == Cameras == | ||
| + | |||
| + | 2 x [https://www.ptgrey.com/chameleon3-32-mp-mono-usb3-vision-sonyimx265 Chameleon3] | ||
| + | |||
| + | == Emission Filters == | ||
| + | |||
| + | [https://www.chroma.com/products/parts/et436-20x ET436/20x] | ||
| + | |||
| + | [https://www.chroma.com/products/parts/et505-20x ET505/20x] | ||
| + | |||
| + | == Dichroic == | ||
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube: | Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube: | ||
| Line 16: | Line 27: | ||
A 3mm dichroic does not fit in the thorlab cube we chose. | A 3mm dichroic does not fit in the thorlab cube we chose. | ||
| − | Thorlab Parts: | + | |
| + | == Thorlab Parts: == | ||
| + | |||
30 mm Cage Cube: 1 x | 30 mm Cage Cube: 1 x | ||
| − | https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH | + | 1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=CM1-DCH CM1-DCH] |
Snap-On Plastic Dust Cap: 1 x | Snap-On Plastic Dust Cap: 1 x | ||
| − | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B | + | 1 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1EC2B SM1EC2B] |
SM1 Lens Tube: 2 x | SM1 Lens Tube: 2 x | ||
| − | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03 | + | 2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1L03 SM1L03] |
SM1 Adapter with External C-Mount Threads 2 x | SM1 Adapter with External C-Mount Threads 2 x | ||
| − | https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39 | + | 2 x [https://www.thorlabs.com/thorproduct.cfm?partnumber=SM1A39 SM1A39] |
Magnetic Post Base: 2 x | Magnetic Post Base: 2 x | ||
| − | https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E | + | 2x [https://www.thorlabs.com/thorproduct.cfm?partnumber=PH2E PH2E] |
Revision as of 14:53, 30 August 2018
This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M.
This is to make a two camera CFP/YFP fret imaging microscope.
3D printed Adapter
Two Camera Adapter:
http://a360.co/2IGYe2w
Cameras
2 x Chameleon3
Emission Filters
ET436/20x
ET505/20x
Dichroic
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
A 3mm dichroic does not fit in the thorlab cube we chose.
Thorlab Parts:
30 mm Cage Cube: 1 x
1 x CM1-DCH
Snap-On Plastic Dust Cap: 1 x
1 x SM1EC2B
SM1 Lens Tube: 2 x
2 x SM1L03
SM1 Adapter with External C-Mount Threads 2 x
2 x SM1A39
Magnetic Post Base: 2 x
2x PH2E
Notes:
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.