Difference between revisions of "Two Cam FRET Microscope"
| Line 2: | Line 2: | ||
This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M. | This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M. | ||
| − | This is to make a two camera CFP/YFP fret imaging microscope. | + | This is to make a two camera CFP/YFP fret imaging microscope. This is using a QUAD cude already instead on the microscope that matches the dichroic and emission filters below. |
== 3D printed Adapter == | == 3D printed Adapter == | ||
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2 x [https://www.ptgrey.com/chameleon3-32-mp-mono-usb3-vision-sonyimx265 Chameleon3] | 2 x [https://www.ptgrey.com/chameleon3-32-mp-mono-usb3-vision-sonyimx265 Chameleon3] | ||
| − | == | + | == Excitation Filters == |
[https://www.chroma.com/products/parts/et436-20x ET436/20x] | [https://www.chroma.com/products/parts/et436-20x ET436/20x] | ||
| Line 19: | Line 19: | ||
[https://www.chroma.com/products/parts/et505-20x ET505/20x] | [https://www.chroma.com/products/parts/et505-20x ET505/20x] | ||
| − | == Dichroic == | + | == CM1-DCH Dichroic == |
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube: | Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube: | ||
Revision as of 14:54, 30 August 2018
This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M.
This is to make a two camera CFP/YFP fret imaging microscope. This is using a QUAD cude already instead on the microscope that matches the dichroic and emission filters below.
3D printed Adapter
Two Camera Adapter:
http://a360.co/2IGYe2w
Cameras
2 x Chameleon3
Excitation Filters
ET436/20x
ET505/20x
CM1-DCH Dichroic
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
A 3mm dichroic does not fit in the thorlab cube we chose.
Thorlab Parts:
30 mm Cage Cube: 1 x
1 x CM1-DCH
Snap-On Plastic Dust Cap: 1 x
1 x SM1EC2B
SM1 Lens Tube: 2 x
2 x SM1L03
SM1 Adapter with External C-Mount Threads 2 x
2 x SM1A39
Magnetic Post Base: 2 x
2x PH2E
Notes:
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.