Two Cam FRET Microscope
This is for a simple lens-less two camera setup for a Zeiss Axiovert 200M.
This is to make a two camera CFP/YFP fret imaging microscope. This is using a QUAD cude already instead on the microscope that matches the dichroic and emission filters below.
3D printed Adapter
Two Camera Adapter:
2 x Chameleon3
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
A 3mm dichroic does not fit in the thorlab cube we chose.
30 mm Cage Cube: 1 x
1 x CM1-DCH
Snap-On Plastic Dust Cap: 1 x
1 x SM1EC2B
SM1 Lens Tube: 2 x
2 x SM1L03
SM1 Adapter with External C-Mount Threads 2 x
2 x SM1A39
Magnetic Post Base: 2 x
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.