Two Cam FRET Microscope

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Revision as of 19:18, 30 August 2018 by Kdb (talk | contribs) (→‎Cameras)
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This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already installed on the microscope that matches the dichroic and excitation filters below. Additional emission filter can be installed in front of each camera.

3D printed Adapter

Two Camera Adapter:


2 x Chameleon3

Why the Chameleon3?

Resolution 2048x1536
FPS 55
QE 71% at 525nm
Dark Noise (e-) 2.89
Price $465

Camera Heat Sinks


These will require slight machining.

4 x M.2 22 X 110 SSD Aluminum Heat sink

Picture on the right is of two Chameleon3s. The top one has the heat sink installed, and other does not

Excitation Filters



Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:

25.5 x 26 x 2mm  Dichroic from Chroma:

NOTE: A 3mm dichroic does not fit in the CM1-DCH cube.

Thorlab Parts

30 mm Cage Cube:

1 x CM1-DCH

Snap-On Plastic Dust Cap:

1 x SM1EC2B

SM1 Lens Tube:

2 x SM1L03

SM1 Adapter with External C-Mount Threads:

2 x SM1A39

Magnetic Post Base:

2x PH2E


Distance from microscope to C-Mount is 61.21 mm.

Add 5 mm since using CS-mount camera rather than C-mount camera.

Total distance should be 66.21 to mounting flange of CS-mount camera.

Current distance in model is 64.772mm, which gives 1.438 mm of space

3.119 mm of room between mount and cube.