Two Cam FRET Microscope
This system is under development and will change.
This is to make a simple lenless two camera CFP/YFP fret imaging Zeiss Axiovert 200M microscope. A QUAD cube already installed on the microscope that matches the dichroic and excitation filters below. Additional emission filter can be installed in front of each camera.
3D printed Adapter
Two Camera Adapter:
2 x Chameleon3
Why the Chameleon3?
|QE||71% at 525nm|
|Dark Noise (e-)||2.89|
Camera Heat Sinks
These will require slight machining.
Picture on the right is of two Chameleon3s. The top one has the heat sink installed, and other does not
Need an "Imaging Splitting" Dichroic for the Thorlab CM1-DCH cube:
25.5 x 26 x 2mm Dichroic from Chroma: https://www.chroma.com/products/parts/t425lpxr
NOTE: A 3mm dichroic does not fit in the CM1-DCH cube.
30 mm Cage Cube:
1 x CM1-DCH
Snap-On Plastic Dust Cap:
1 x SM1EC2B
SM1 Lens Tube:
2 x SM1L03
SM1 Adapter with External C-Mount Threads:
2 x SM1A39
Magnetic Post Base:
We are seeing a 1.3mm shift on the X axis between the two cameras. This is due to the dichroic. We are looking at Thorlabs ST1XY-A.
Distance from microscope to C-Mount is 61.21 mm.
Add 5 mm since using CS-mount camera rather than C-mount camera.
Total distance should be 66.21 to mounting flange of CS-mount camera.
Current distance in model is 64.772mm, which gives 1.438 mm of space
3.119 mm of room between mount and cube.