Difference between revisions of "VESM"
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(Created page with " == Microscope Alignment == === Sideport Alignment === The Filter Carousel should be removed. A pen laser is set up on a vertical mount mounted on tghe stage. The laser sho...") |
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Align beam to center of target. | Align beam to center of target. | ||
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| + | === Andor Zyla 4.2 === | ||
| + | |||
| + | {| class="wikitable" | ||
| + | !TRIGGER MODE | ||
| + | !FPS | ||
| + | |- | ||
| + | |External | ||
| + | |50.2978 | ||
| + | |- | ||
| + | |External Exposure | ||
| + | |99.968 | ||
| + | |- | ||
| + | |External Start | ||
| + | |50.3951 | ||
| + | |- | ||
| + | |Internal | ||
| + | |50.3951 | ||
| + | |- | ||
| + | |Software | ||
| + | |50.2978 | ||
| + | |} | ||
| + | Trigger | ||
| + | FPS | ||
Latest revision as of 15:42, 21 April 2016
Microscope Alignment
Sideport Alignment
The Filter Carousel should be removed.
A pen laser is set up on a vertical mount mounted on tghe stage. The laser should centered directly over a ground glass disk with a pin hole that is attached to the objective mount.
Adjust the microscope until center of beam is aligned with target.
Backport Alignment
The Filter Carousel should be installed, and #1 cube should be used.
Install an 8" vertical rod and a small thread rod on top. Only microscope side to side movement should be done.
Recheck Sideport alignment (sans Filter Carousel).
TuCam Alignment
Align the straight-through port, without cameras attached.
Do not have the TuCam dichroic in place.
Use filter cube 1 in the Filter Carousel.
Align beam to center of target.
Andor Zyla 4.2
| TRIGGER MODE | FPS |
|---|---|
| External | 50.2978 |
| External Exposure | 99.968 |
| External Start | 50.3951 |
| Internal | 50.3951 |
| Software | 50.2978 |
Trigger FPS