The Filter Carousel should be removed.
A pen laser is set up on a vertical mount mounted on tghe stage. The laser should centered directly over a ground glass disk with a pin hole that is attached to the objective mount.
Adjust the microscope until center of beam is aligned with target.
The Filter Carousel should be installed, and #1 cube should be used.
Install an 8" vertical rod and a small thread rod on top. Only microscope side to side movement should be done.
Recheck Sideport alignment (sans Filter Carousel).
Align the straight-through port, without cameras attached.
Do not have the TuCam dichroic in place.
Use filter cube 1 in the Filter Carousel.
Align beam to center of target.